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Part:BBa_K1189006:Design

Designed by: Sharon Feng and Julie Osgood   Group: iGEM13_Calgary   (2013-09-16)

TALEA and TALEB Target


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 213
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Once the individual target sequences were made, we designed new primers to create a target sequence construct with both the target sequences together. We did a KAPA PCR to produce our linear product. The PCR product consisted of the two target sequences and cut sites for EcoR1, Xba1 and Spe1. We then ligated our linear PCR product into a pSB1C3 backbone by switching out RFP.


Source

Primers

References

  • Bogdanove, A. J., Schornack, S., & Lahaye, T. (2010). TAL effectors: finding plant genes for disease and defense. Current Opinion in Plant Biology, 13(4), 394–401. doi:10.1016/j.pbi.2010.04.010
  • Mussolino, C., & Cathomen, T. (2012). TALE nucleases: tailored genome engineering made easy. Current Opinion in Biotechnology, 23(5), 644–50. doi:10.1016/j.copbio.2012.01.013